![]() When faced with love, 4 becomes honest and serene, being able to express their feelings freely and show their true colors. ![]() The purpose behind the energy of number 4 is to get organized and start making things happen and turning dreams into reality. There is a remarkable capacity to undertake complex tasks and remain focused for a long time and are even bent on self-sacrificing. The number 4 supports the idea of putting ideas into form and harnesses productivity and an increased sense of responsibility. Click publication title for the full text.Blessed Careers: Aerospace, Forestry, Fitness or similar. Please click the arrow on the right to expand the citation list. Related products: Universal Restriction Buffer, #EN-300 Supercoiled plasmids may require up to 5-fold more NotI for complete digestion than linear DNA.Īll preparations are assayed for contaminating endonuclease, 3'-exonuclease, 5' exonuclease/ 5' phosphatase, as well as nonspecific single- and doublestranded DNase activities. To obtain best results, consult the corresponding manuals of all involved products.Īfter 25-fold overdigestion with NotI, >98% of the DNA fragments can be ligated and recut with this enzyme. Our restriction enzymes are fully compatible to restrictases and buffer systems from other manufacturers and can be used along in double digestions. If optimum condition for second enzyme is different than the recommended for the first enzyme, we suggest carrying out first the restriction at the higher recommended concentration of UB and dilute the reaction volume to the adequate UB concentration for further proceeding with the second restriction. Within the Universal Buffer (UB) system, the most majority of our enzymes display 100% Relative Activity in 1x UB and only few either in 0.5x or 2x UB. Please note that the optimum digestion condition for this enzyme is 1x UB. Phenol-Chloroform Extraction or Ethanol Precipitation.ġx UB - 100 % Relative Activity (recommended) ![]() Gel Electrophoresis and Single Band Excision (e.g. Addition of 2.1 μl EDTA pH 8.0, final 20 mM After enzyme addition, mix gently by pipetting. Mix components well before adding enzyme. The enzyme should not exceed 10 % of total reaction volume.plant genomic DNA) may require longer incubation time or higher amount of enzyme.Ģ Some enzymes may require additional DNA bases flanking the restriction site for complete digestion. RNA Labeling Selector (Kits & Nucleotides)ġ Supercoiled or high molecular weight DNA (e.g.DNA Labeling Selector (Kits & Nucleotides).Jena Bioscience at Meetings, Conferences and Fairs. ![]() Privacy Policy for Jena Bioscience's Facebook Page.Poly (A) carrier RNA-based RNA purification.3'-End RNA Labeling (T4 RNA Ligase 1-based).Random RNA Labeling ( in vitro Transcription-based).Click Chemistry-based RNA/cRNA Labeling.Click Chemistry-based DNA/cDNA Labeling.Quantifoil® Holey Carbon Films with 2 nm Continuous Carbon.Mercurated and Selenium-containing Nucleotides.JBS Tungsten Cluster Derivatization Kits.Cryo and Room Temperature Crystallography.in Posttranslational Modification Analysis Immobilized Nucleotides for Affinity Chromatography.on Proteins/Enzymes - Sulfonyl Fluoride Probes Cancer and Proliferation Marker Nucleosides.
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